HOECHST 33258

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Name：	HOECHST 33258
CAS：	23491-44-3 ;23491-45-4
Synonyms：	HOECHST 33258,98% ; HOECHST 33258 ; BIS-BENZIMIDE ; PIBENZIMOL ; BISBENZIMIDE H 33258 ; HOECHST STAIN
MDL.：	MFCD00044760
H bond acceptor：	7
H bond donor：	3
Smile：	CN1CCN(CC1)c2ccc3c(c2)nc([nH]3)c4ccc5c(c4)nc([nH]5)c6ccc(cc6)O
InChi：	InChI=1S/C25H24N6O/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29)
InChiKey：	InChIKey=INAAIJLSXJJHOZ-UHFFFAOYSA-N
Property
Comments：	COMMENT 1: CONVENTIONAL DYES CANNOT BE USED IN IMMUNOFLUORESCENT-STAINED TISSUES BECAUSE THEY OFTEN CONTRIBUTE TO BACKGROUND FLUORESCENCE COMMENT 2: HOWEVER, DNA MARKER DYES SUCH AS BIS-BENZIMIDE (HOECHST 33258) AND DAPI STAIN A PALE BLUE WHEN VIEWED WITH A FILTER SET IN THE ''TRUE BLUE'' EMISSION RANGE COMMENT 3: THESE DYES ARE COMPATIBLE WITH BOTH FLUORESCEIN AND RHODAMINE LABELS FOR COUNTERSTAINING DNA COMMENTS: SOLUBLE IN H2O AND DMF COMMENTS: SUPRAVITAL MINOR GROOVE-BINDING DNA STAIN COMPATIBLE BLUE COLOR WITH FLUORESCENT STAINING EMISSION WAVELENGTH (NM): 480 EXCITATION WAVELENGTH (NM): 343 FLUORESCENT SITE-SELECTIVE PROBE
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